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Immunohistochemistry (IHC) demonstrated positivity for S100 , negativity for Melan-A and HMB45 , and a Ki-67 index of 0%

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MIF null mice are entirely resistant to the detrimental effects of OVX, implying that MIF acts as a key downstream mediator of both the detrimental effects of estrogen deficiency and the beneficial effects of estrogen replacement on skin wound healing [57, 175, 182]

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