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The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L 1 NaCl at 12 C) and quenched in cold methanol/water (1:1 v/v) at 30 C as described by Smart et al

The fat triggers endogenous bile release before the injection, blunting the secondary bile surge from methionine metabolism

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Arch Cardiovasc Dis 105(67):373385 Wen H, Gwathmey JK, Xie LH (2012) Oxidative stress-mediated effects of angiotensin II in the cardiovascular system

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In addition, GLP-1 therapies may modify bile acid metabolism, which is known to shape microbial diversity and metabolic signaling pathways