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Mast cells are increased in the synovial compartments in RA patients and synovial fibroblasts in AIA produce IL-33, which activates mast cells to release inflammatory mediators including IL-17, IL-13, GM-CSF, MCP-1, and MIP-1 (Robbie-Ryan and Brown, 2002

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Researchers must avoid repeated freeze-thaw cycles, as these temperature fluctuations promote peptide degradation

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