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Trial participants needed to have a BMI of 30 kg/m 2 or a BMI of 27 kg/m 2 plus a previous diagnosis of 1 or more weight-related comorbidities defined as hypertension, dyslipidemia, obstructive sleep apnea, or cardiovascular disease

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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h