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Melanin is the primary determinant of skin pigmentation and hair color, and if you produce too much melanin in any area of your body, the result is hyperpigmentation, a brown spot, or discoloration on your skin

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2.4.4 Sample preparation and analysis Briefly, each sample (400 L) containing the IS metacycline (2.5 g/mL) was mixed with acetonitrile (790 L), vortexed thoroughly for 2 min, and centrifuged at 12,000 rpm, 4 C for 10 min

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