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This usually peaks around days 2 to 3 after each injection and fades by day 5

After alcohol administration, 10 responses on the alcohol-appropriate lever (e.g., correct lever) resulted in sucrose delivery, while responses on the other (e.g., incorrect lever) produced no programmed consequence

developed a software-based technique, which allowed performing morphometric analysis of eryptotic cells evaluating cell size (cell shrinkage), cell irregularities (membrane blebbing), granularity, and central halo (loss of a biconcave shape) [85]

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To this end, the total RNA was isolated using the Total RNA Purification Kit (NorgenBiotek Corp., Ontario, CA, USA) according to the manufacturers instructions