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381 Besides, high-fiber diet and acetate supplementation can lead to changes in the gut microbiota, particularly an increase in Bacteroides acidifaciens , which is protective against the development of CVD

We later followed up with young distributors

10.1074/jbc.M109.020107 37 DixonD

40 l of supernatant was removed from each treated well and transferred into a 96-well clear bottom black microplate (Costar, Corning, USA)

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The reaction mixture (1 ml) containing Lockes buffer (pH 7.4), 0.2 ml homogenate and 10 l of DCFH-DA (5 mM) was incubated for 45 min at room temperature and the fluorescence intensity of DCF was recorded by excitation at 485 nm and emission at 535 nm using a Synergy TM 2 Multi-function Microplate Reader (Bio-Tek Instruments Inc., Winooski, Vermont, USA)