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Immunohistochemistry (hair cell viability)Fixed and dissected cochleae are incubated in PBS-T with 10% normal goat serum with a phalloidin primary antibody overnight at 4 C

Unlike conventional sedatives, DSIP tends to normalize sleep architecture without next-day grogginess

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Additionally, BPC-157 activates signal pathways involving Src, caveolin-1 (Cav-1), and eNOS, while reducing protein-protein interaction between eNOS and Cav-1, thereby enhancing nitric oxide bioavailability

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