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A 32 min gradient using 10 mM ammonium acetate in methanol with 0.1% formic acid (mobile phase A), 10 mM ammonium acetate in isopropanol with 0.1% formic acid (mobile phase B) and 10 mM ammonium acetate in HPLC grade water with 0.1% formic acid (mobile phase C) was used

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